Figure 5.

Figure 5. Most significantly altered metabolites between WT and MUT1 larvae based on untargeted metabolomics.

(A) Volcano plot (corrected P-value versus fold change ratio) showing significantly (P < 0.001) altered metabolites in red for experiment 1. Each dot represents one metabolite. NAAG (previously reported as a potential NCL biomarker (33)) is highlighted in blue and GPD species in turquoise. (B, C, D, E) Violin plots showing levels of selected differential metabolites as abundance values, normalized as described in the Materials and Methods section. Statistically significant differences between the WT and MUT1 lines were determined using an unpaired multiple Welch’s t test (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; and ****P < 0.0001) (n = 240 for each line, extracted and analyzed in batches of 40).