ZFIN Image: Hall et al., 2023, Figure 3

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Figure 3

Confirmation of decreased Mgat1b expression, and an increase in oligomannose N-glycans in mutant strain of adult and larvae fish. Lectin blots of whole cell lysates of adult brain (A), and larvae at 24 dpf and 6 dpf from Wt AB and Mgat1b^−/− mutant zebrafish (B). Separated proteins were probed with Phaseolus vulgaris Erthroagglutinin (E-PHA) or Galanthus nivalis lectin (GNL). Coomassie blue (CB)-stained gel demonstrating equal protein loads among the samples. Lines adjacent to the blots denote protein markers (in KDa) 250, 150, 100, 75, 50, 37. Bar graphs represent Mgat1b mRNA levels from Wt AB and Mgat1b^−/− mutant zebrafish relative to housekeeping genes β-actin (C) and ELFα (Figure S1). Data are presented as mean ± SEM, n ≥ 3 and were all compared using Student’s t-test (** p < 0.005, * p < 0.02).

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Iss. Mol. Biol.