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Fig. 8 Synaptic mitochondrial activity is reduced in plectin morphants. A-H: 3dpf Tg(NeuroD-GFP) live morphants were incubated with Mitotracker as a proxy for mitochondria activity. A-B: Scrambled animals. C-D: plectin a morphants. E-F: plectin b morphants. G-H: plectin a + b morphants. Mitotracker (red). GFP (green). B’, D’, F’ and H’: Z-view high magnification images of the base of the hair cells from the different groups. Scale bar: A-H: 5 μm. B’, D’, F’, H’: 2 μm. I: Violin plots comparing the total fluorescence intensity (% from control) of the incorporated Mitotracker in the different groups. Each dot represents the total fluorescence per neuromast, with at least two neuromasts inspected per fish, and between 6 and 10 fish inspected. One-way ANOVA followed by Dunnett T3 multiple comparisons test.
Reprinted from Hearing Research, 436, Zhang, T., Xu, Z., Zheng, D., Wang, X., He, J., Zhang, L., Zallocchi, M., Novel biallelic variants in the PLEC gene are associated with severe hearing loss, 108831108831, Copyright (2023) with permission from Elsevier. Full text @ Hear. Res.