Fig. 5

Fig. 5 Spatiotemporal distribution of plectin in mouse inner ear. A-R: Representative images from at least three different animals at each time point. Cochlea cross sections of the organ of Corti at E16 (A-B), P1 (E-F), P7 (K-L) and P33 (O-P). Cochlea cross-sections of the spiral ganglion neurons at E16 (C-D), P1 (G-H, G’-H’), P7 (M-N, M’-N’) and P33 (Q-R). G’-H’ and M’-N’ correspond to higher magnification images of the framed areas in H and N, respectively. I-J: Whole mount P1 organ of Corti. Insert in J represent a Z-view of the outer hair cell apical structures. Arrowheads denote plectin immunopositive staining. Asterisks in K denote neuronal fiber staining. Plectin (plec, red), myosin 7A (Myo7A, yellow), beta-III tubulin (Tuj1, green), DAPI (blue). Scale bars: A-R, G’-H’, and M’-N’: 10 μm. Inset in J: 2 μm. S-T: Immunoblot analysis of the organ of Corti and spiral ganglion at the different developmental time points under study. S: Representative immunoblot. Numbers (left) indicate molecular weight markers. Total protein was used as a loading control. T: Quantification of at least 3 independent experiments. Results are presented as mean +/- SEM. OHC: outer hair cells. IHC: inner hair cells