Fig. 3

Zebrafish rerea homozygous mutants exhibit coloboma. Three-day post fertilization WT zebrafish embryos (A, B) exhibit fused optic fissure margins (54 genotyped embryos), whereas rerea homozygous mutants exhibit coloboma (E, F, 52/54 genotyped embryos). Sagittal histological sectioning of WT (C, D, N = 10 genotyped embryos) and rerea mutant (G, H, N = 10 genotyped embryos) zebrafish eye (3 dpf). The schematic in C gives the approximate plane of sectioning for the histologic sections. Coloboma showed variable penetrance in embryos at 5 dpf (I). Measurement of eye area (lateral view, Figure 2K) revealed significantly smaller eye at 3 dpf (P < .05) of rerea mutant larvae compared with WT. No statistical difference in eye size was observed at 5 dpf (J). Student's t test was used to determine level of significance and P value is provided (N = 30 genotyped embryos/group).