Fig. 7

Reduced assembly of mitochondrial Complex I in chchd10^−/− and chchd2^−/− zebrafish larvae, but not in double chchd10^−/− and chchd2^−/− larvae at 5 dpf. (a) Immunoblot analysis of mitochondrial respiratory chain subunits in larvae aged 5 dpf and quantification (b). Ndufa9 is significantly reduced in chchd10^−/− larvae (p = .003). Data is represented as mean ± SEM. Sample sizes (n) are noted in parentheses. (c) Representative blue native PAGE with each lane constituted of an individual 5 dpf larvae of respective genotype and quantification (d). Significant reduction of Complex I assembly was observed in chchd10^−/− larvae (p = .003), and chchd2^−/− larvae (p = .044), but not in double chchd10^−/− and chchd2^−/− model (p = .990). Significance was assessed using a one-way ANOVA and Tuckey's multiple comparison test, and significant differences from wild type larvae are represented by either a single asterisk (p < .05) or double asterisk (p < .01). Data is represented as mean ± SEM. Sample sizes (n) are noted in parentheses.