Fig. 6

Both Chchd10 and Chchd2 proteins display elevated expression levels in response increased mitochondrial reliance in wild type larvae. (a) Experimental timeline for 2-DG treatment (images were created using BioRender). (b) Example immunoblot showing Chchd10 and Chchd2 expression change following treatment with to 2-DG. (ci, ii) Quantification of immunoblot data. Significant difference in both Chchd10 (t-test, p = .05), and Chchd2 (t-test, p = .04) protein levels following 2-DG treatment was observed. Vinculin was used as loading control. (ciii, iv) Unchanged expression levels following 2-DG exposure in both transcripts: chchd10 (t-test, p = .59, relative to actin) and chchd2: (t-test, p = .78, relative to actin). (di) Representative touch-evoked larval motor responses following exposure to 2-DG or E3 buffer treatment for 24 h. Motor responses were evaluated at 56 hpf. (dii) Larvae exposed to 2-DG show significantly reduced swim distance (t-test, p = .04), though maximum swim velocity. Part (diii) was not found to be significantly different (t-test, p = .80). Sample sizes (n) are noted in parentheses. Single asterisks represent significant differences from E3-treated wild type larvae (p < .05).