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Fig. 7 Ccn2a interacts with Fgfr1a and Fgfr1b in vivo. (A) Schematic of experimental procedures. (B,C) Representative single plane optical sections of whole-mount 30 hpf embryos stained for proximity detection (cyan, interacting complex) and nuclei (white). Arrowheads in B indicate an interaction between C-terminal Myc-tagged Ccn2a and N-terminal FLAG-tagged Fgfr1a (cyan). Arrows in C indicate an interaction between N-terminal Myc-tagged Ccn2a and N-terminal FLAG-tagged Fgfr1b (cyan). (D) Model of the signaling cascade regulated by Ccn2a, which promotes cell proliferation and inhibits cell death.
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