Figure 6

Sinusoids are reduced in the jam1a^sd43 kidney

(A) Dorsal view of a jam1a^sd43kdrl:Cerulean kidney. DA, dorsal aorta.

(B) Transverse section of a jam1a^sd43kdrl:Cerulean kidney. Blue areas outlined by the white dotted line denote the sinusoidal area. AF in A and B denotes auto-fluorescence of renal tubules.

(C) Percentages of sinusoidal area in the wild-type or jam1a^sd43 kidney. Error bars, s.d. (n = 6 for each).

(D) Schematic diagram of qPCR analysis in vascular endothelial cells from wild-type or jam1a^sd43 kidneys.

(E) Expression of niche factor genes in vascular endothelial cells from wild-type or jam1a^sd43 kidneys. Error bars, s.d. (n = 4 for each).

(F) Representative result of FCM analysis in wild-type kdrl:Cerulean animals injected with Alexa Fluor 488-conjugated Ac-LDL. kdrl:Cerulean⁺ endothelial cells were subdivided into two populations, Ac-LDL^high and Ac-LDL^low.

(G and H) Expression of jagged-1a in Ac-LDL^high or Ac-LDL^low endothelial cells of wild-type animals (G) or in Ac-LDL^high endothelial cells from wild-type or jam1a^sd43 kidneys (H). Error bars, s.d. (n = 4 for each). u.d., undetected. Bars, 200 μm (A); 40 μm (B); ∗∗p < 0.01; ∗∗∗p < 0.001.