Fig. 6

Photomicrographs of transverse double-labeled sections through hypothalamic and diencephalic areas of Protopterus dolloi (P.d), Polypterus senegalus (P.s), Acipenser ruthenus (A.r), Danio rerio (D.r), and Lepisosteus oculatus (L.o) showing the location of SATB1/2 or SATB1 (green color) alone or in combination with Otp or TH (red color) immunoreactivity. The markers and the species are indicated in each image. a–c SATB1/2 labeling in cells of the subparaventricular hypothalamic region (a, b) and the prethalamic and thalamic areas (c) of P. dolloi. d SATB1/2 cells in the rostral part of the subparaventricular region of P. senegalus. e, f Immunoreactive cell nuclei in the preoptic area and the subparaventricular region of A. ruthenus. g SATB1/2-ir cells in the subparaventricular region of D. rerio; empty arrowheads point to double labeled cells. h Labeled cells in the prethalamus within the alar prosomere 3 of the lungfish. i Tuberal hypothalamic region of P. senegalus showing SATB1-ir cells. j SATB1 labeling in the prethalamus and posterior tubercle of the sturgeon. k, l SATB1 immunoreactivity in cells of the thalamus and pretectum of L. oculatus (k) and P. senegalus (l). m SATB1-ir cells in the mamillary region of P. senegalus; empty arrowhead points to double labeled cells. n–q Labeled cells in the posterior tubercle (n) and the tuberal hypothalamic region (o–q) of the lungfish; empty arrowheads in q point to double labeled cells. For abbreviations, see list. Scale bar: a–k, n–t = 200 µm; m, l = 100 µm; u = 50 µm