ZFIN Image: Fazal et al., 2023, FIGURE 2

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Figure Caption

FIGURE 2

sarm1 expression in zebrafish larvae PLLn and spinal cord. (A) MAX projection, lateral view of PLLn of a 5dpf larva demonstrating HCR in situ labeling for sox10 and sarm1 mRNA and DAPI staining to mark nuclei. (B) Single confocal z plane, 616 nm optical thickness, lateral view of PLLn of a 5dpf larva. Arrowheads mark nuclei, labeled with DAPI, positive for sox10 and sarm1 mRNA. In the PLLn, 22.4% ± 3.161 [mean and standard error of mean (SEM), n = 5 larvae, 5 sections per larvae] of sox10 positive cells also expressed sarm1. (C) MAX projection, lateral view of spinal cord of a 5dpf larva demonstrating HCR in situ labeling for sox10 and sarm1 mRNA and DAPI staining to mark nuclei. Arrowheads mark nuclei positive for sox10 and sarm1 mRNA. (D) Single confocal z plane, 475 nm optical thickness, lateral view of spinal cord of a 5dpf larva. Arrowheads mark nuclei positive for sox10 and sarm1 mRNA. In the spinal cord, 60.3% ± 2.4 (mean and SEM, n = 5 larvae, 3 sections quantified per larvae) of sox10 positive cells also expressed sarm1. For all images: sox10 mRNA (magenta) and sarm1 mRNA (green), nuclei labeled with DAPI (gray), scale bar 25 μm.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.