ZFIN Image: Li et al., 2023, Figure 3

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Figure Caption

Figure 3

Cu overload induces HSPC proliferation impairment

(A) Cell cycle of HSPCs in Cu-stressed embryos at 33 hpf. A1, Schema for the experiments; A2, flow cytometry (FACS) histogram; A3, cell cycle stage calculation.

(B) Mitotic malformation of HSPCs in Cu-stressed embryos at 33 hpf (AGM) and 72 hpf (CHT), respectively. B1, B5, B10, B14, DAPI staining; B2, B6, B11, B15, anti-α-tubulin staining; B3, B7, B12, B16, anti-GFP staining; B4, B8, B13, B17, merged. At least 10 mitotic HSPCs in more than 10 embryos were observed for each group. B9, B18, calculation the length of spindles in metaphase runx1GFP⁺ cells at 33 hpf and 72 hpf, respectively.

(C) BrdU cell proliferation assays in embryos at 33 hpf (C2-C9) and 72 hpf (C12-C19), respectively. C1, C11, AGM and CHT domain in embryos, respectively; C10, C20, calculation of the number of proliferative HSPCs in embryos from different groups. C2-C9, C12-C19, lateral view, anterior to the left, and dorsal to the up. Data are mean ± SD. t-test, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. NS, not significant. Scale bars, 2 μm (B1-B8, B10-B17) and 20 μm (C2-C9, C12-C19).

Acknowledgments

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