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Figure 3
(A?B) Ciliary beating frequency decreases under increasing viscosity conditions (0?2% methylcellulose) and partially recovers upon re-exposure to 0% methylcellulose (0%*). (A) Representative example of ciliary beat frequency (CBF) maps of a 4-day-old zebrafish nose. (B) CBF for n=9 (gray) and average in black. A repeated measures ANOVA (*) indicates a significant effect of viscosity conditions on CBF (p = 0.003; n = 9). (C?D) Ciliary coherence extends with increased fluid viscosity. (C) A representative example of pairwise coherence versus distance for different viscosity conditions (Coherence bin width = 0.04/bin; distance bin width = 0.5 µm/bin). (D) Mean coherence across distance bins (width = 0.5 µm) for different viscosity conditions shows that coherence domains expand for conditions of increased viscosity. Mean curves and standard error of the mean are plotted (n=9). ANOVA-N indicates a significant effect of viscosity conditions on mean coherence across distances (p=3?10-5). CBF = Ciliary Beat Frequency.
An increase in fluid viscosity decreases ciliary beat frequency and extends the spatial range of cilia coherence.