Image
|
Figure Caption
Fig. 6
The serotonergic system. A and B, Ventral views of whole-mount 6 dpf larval brains, anterior to the top, processed for serotonin (5-HT) immunostaining. 5-HT immunoreactivity in the brain of a vmat2+/+ larva and clear depletion of 5-HT immunoreactive neurons on its vmat2−/− sibling brain. Scale bar = 75 µm. C and D, Ventral views of whole-mount 6 dpf larval brains, anterior to the top, processed for tph1a RNA in situ hybridisation (ISH). More tph1a-positive neurons are seen in the hypothalamus of a vmat2−/− larva than in vmat2+/+ sibling, particularly on group 3 (dashed box). Scale bar = 75 µm. E and F, Bar chart showing results of high-performance liquid chromatography (HPLC) assay on larvae of the indicated genotype at 6 dpf. Levels of 5-HT and 5HIAA in 6 dpf vmat2+/+ and vmat2−/− larvae. n = 6 for each genotype. Data are mean ± SEM. Student's t test was used for statistical analysis. †P < .01. ‡P < .001
Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Acta Physiol. (Oxf).