Fig. 9

a Representative images of pS6(240/244) and GFP immunofluorescence in islet sections of control, gcgr^DKO and casr−/−;gcgr^DKO larvae at 5 dpf. The pS6(240/244) signal in α cells is indicated by arrows, primary antibody: Anti-pS6 (Ser240/244) (1:300, rabbit); secondary antibody: Alexa Fluor 568 (1:1000, goat anti-rabbit) (scale bar, 7 μm). b Quantification of the percentage of pS6(240/244) positive α cells in control, gcgr^DKO, and casr−/−;gcgr^DKO larvae at 5 dpf (data represent the means ± SD, n = 9 for each group). c Quantification of the pS6(240/244) fluorescence intensity in α cells of control, gcgr^DKO and casr−/−;gcgr^DKO larvae at 5 dpf (data represent the means ± SD, n = 9 for each group). d qPCR analysis of Slc38a5 in mouse islets cultured in LAA and HAA media with or without 0.5 µM NPS 2143 for 4 days. (data represent the means ± SD, n = 4 for each group). e Representative images of pS6(240/244) and GFP immunofluorescence in islet sections of control and gcgr^DKO larvae treated with 10 μM U0126 or vehicle for 48 h at 5 dpf. The pS6(240/244) signal in α cells is indicated by arrows, primary antibody: Anti-pS6 (Ser240/244) (1:300, rabbit); secondary antibody: Alexa Fluor 568 (1:1000, goat anti-rabbit) (scale bar, 7 μm). f Quantification of the percentage of pS6(240/244) positive α cells in control and gcgr^DKO larvae treated with 10 μM U0126 or vehicle for 48 h at 5 dpf (data represent the means ± SD, n = 9 for each group). g Quantification of the pS6(240/244) fluorescence intensity in α cells of control and gcgr^DKO larvae treated with 10 μM U0126 or vehicle for 48 h at 5 dpf (data represent the means ± SD, n = 9 for each group). *P < 0.05, ***P < 0.001, ****P < 0.0001, NS indicates no significant difference (one-way ANOVA, Tukey’s multiple comparisons test, the quantifications represent individual islet sections). Source data are provided as a ^{Source Data} file.