ZFIN Image: Brandt et al., 2021, Fig. 7

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Figure Caption

Fig. 7

Collagen fibril diameter in mfrp⁺^/^? and mfrp^?^/^? sclera with and without macrophage ablation. Representative examples of collagen fibrils in the central posterior sclera of mfrp⁺^/^?; mpeg1:NTR-eYFP⁺ (A) and mfrp^?^/^?; mpeg1:NTR-eYFP⁺ (A') untreated fish. (B) Frequency distribution of collagen fiber diameter with y-axis = relative frequency as a percentage and x-axis = collagen fiber diameter in 5-nm bins. mfrp⁺^/^? is indicated by a green line, mfrp^?^/^? by a purple line. For mfrp⁺^/^?, n = 3; for mfrp^?^/^?, n = 4. (C) Representative examples of collagen fibrils in the central posterior sclera of mfrp⁺^/^?; mpeg1:NTR-eYFP⁺ (C) and mfrp^?^/^?; mpeg1:NTR-eYFP⁺ (C') MTZ treated fish. (D) Frequency distribution of collagen fiber diameter with y-axis = relative frequency as a percentage and x-axis = collagen fiber diameter in 5-nm bins. mfrp⁺^/^? is represented by a blue line, mfrp^?^/^? by a yellow line. For mfrp⁺^/^?, n = 5; for mfrp^?^/^?, n = 5. Multiple t-tests were used for statistical analysis. *P < 0.001. (E) Combined graph of (B) and (D). Errors bars represent standard deviations throughout figure. Two-way ANOVA with Tukey's multiple-comparisons were used for statistical analysis. An asterisk (*) denotes a significant difference between mfrp⁺^/^? MTZ treated and mfrp^?^/^? MTZ treated; a plus sign (+) denotes a significant difference between mfrp⁺^/^? MTZ treated and mfrp⁺^/^? untreated; and the pound symbol (#) denotes a significant difference between mfrp⁺^/^? MTZ treated and mfrp^?^/^? untreated. P < 0.05 for all significant differences in (E).

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Acknowledgments

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