Fig. 7

Collagen fibril diameter in mfrp^+/– and mfrp^–/– sclera with and without macrophage ablation. Representative examples of collagen fibrils in the central posterior sclera of mfrp^+/–; mpeg1:NTR-eYFP⁺ (A) and mfrp^–/–; mpeg1:NTR-eYFP⁺ (A') untreated fish. (B) Frequency distribution of collagen fiber diameter with y-axis = relative frequency as a percentage and x-axis = collagen fiber diameter in 5-nm bins. mfrp^+/– is indicated by a green line, mfrp^–/– by a purple line. For mfrp^+/–, n = 3; for mfrp^–/–, n = 4. (C) Representative examples of collagen fibrils in the central posterior sclera of mfrp^+/–; mpeg1:NTR-eYFP⁺ (C) and mfrp^–/–; mpeg1:NTR-eYFP⁺ (C') MTZ treated fish. (D) Frequency distribution of collagen fiber diameter with y-axis = relative frequency as a percentage and x-axis = collagen fiber diameter in 5-nm bins. mfrp^+/– is represented by a blue line, mfrp^–/– by a yellow line. For mfrp^+/–, n = 5; for mfrp^–/–, n = 5. Multiple t-tests were used for statistical analysis. *P < 0.001. (E) Combined graph of (B) and (D). Errors bars represent standard deviations throughout figure. Two-way ANOVA with Tukey's multiple-comparisons were used for statistical analysis. An asterisk (*) denotes a significant difference between mfrp^+/– MTZ treated and mfrp^–/– MTZ treated; a plus sign (+) denotes a significant difference between mfrp^+/– MTZ treated and mfrp^+/– untreated; and the pound symbol (#) denotes a significant difference between mfrp^+/– MTZ treated and mfrp^–/– untreated. P < 0.05 for all significant differences in (E).