(A–D) Lateral views of microcomputed tomography (µCT) reconstitutions of wild-type (A), cfap298tm304 mutants (B), urp1∆P;urp2∆P double mutants (C), and cfap298tm304;urp1∆P;urp2∆P triple mutants (D). All fish shown are female. Scale bar: 10 mm. (E) Total Cobb angles with each circle representing an individual fish. The mean ± s.d. is shown. p-Values are given from two-tailed unpaired student’s t tests. U — urp1∆P;urp2∆P double mutants; C — cfap298tm304 mutants; UC — cfap298tm304;urp1∆P;urp2∆P triple mutants. (Fi) Dorsal views of µCT reconstitutions with ribs and fins removed. Scale bar: 5 mm. (Fii) Quantitation of degree of lateral curvature for wild type (n=5) and urp1∆P;urp2∆P (n=8), uts2r3b1436 (n=3), cfap298tm304 (n=3), and cfap298tm304;urp1∆Purp2∆P (n=6) mutants. y-axis is the arbitrary units.
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