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Fig 5
Candidate genes are required for KV formation and ciliogenesis.
(A) The light-micro graph at the 8-somite stage showed different KV sizes, including normal and small. (B) The vesicle size of KV apical area. N≥19. (C) Fluorescent immunostaining of cilia in KV using anti-α-Tubulin antibodies at the 12hpf. (D) LRO Cilia number per KV was quantified and the group values were expressed as the mean ± SD. N≥15. (E) The average length of LRO Cilia per KV was quantified and the group values were expressed as the mean ± SD. N≥7. (F) The ciliary beat frequency (CBF). N≥12. Statistical significance was determined by Mann Whitney test; *P <0.05, ***P < 0.001, respectively vs. Std.
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