ZFIN Image: Meyer-Miner et al., 2022, Figure 6

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Figure Caption

Figure 6

Analysis of Sspo localization and RF formation in 21dpf katnb1 mutant brains

(A–L) Representative maximum intensity Z-stack projections of confocal micrographs, acquired through dorsally oriented whole mount brains that were dissected from 21dpf katnb1^mh102/+ control (A, C–G; N = 7, n = 54) and katnb1^mh102/mh102 mutant (B, H-L; N = 7, n = 36) fish, and immunostained for polyglutamylated tubulin (green) and Sspo (magenta). Squares indicate regions of interest, chosen for higher magnification analyses of areas surrounding the fChP (C, H); subcommisural organ (SCO; D, I); optic tectum (TeO; E, J); rChP (F, K); and medulla spinalis (G, L). (C–L) Inverted, higher magnification images of Sspo immunostaining. (C–G) Normal Sspo localization is observed in katnb1^mh102/+ control animals, including the presence of a RF (arrows). (H–L) katnb1^mh102/mh102 mutant images, exhibiting abnormal Sspo accumulation at the fChP and rChP (double arrowheads), and the presence of a RF (arrows). Scale bars, 100 μm (A & B) and 50 μm (C–L).

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Phenotype details

Acknowledgments

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