Figure 4

Figure 4

katnb1 mutants exhibit choroid plexus cilia defects at 21 dpf

(A–F) Representative maximum intensity Z-stack projections of confocal micrographs, acquired through dorsally oriented whole mount brains that were dissected from 21dpf katnb1^mh102/+ (A; N = 6, n = 14) and katnb1^mh102/mh102 (D; N = 6, n = 25) fish, and immunostained for polyglutamylated tubulin. Squares indicate regions of interest, chosen for higher magnification analyses of the forebrain ChP (B & E) and rhombencephalic ChP (C & F) of katnb1^mh102/+ control (B & C; N = 8, n = 60) and katnb1^mh102/mh102 mutant (E & F; N = 8, n = 32) animals. Scale bars, 50 μm. Tel: telencephalon, TeO: optic tectum, CC: cerebellum.

(G–J) Higher magnification regions (as indicated in B, C, E & F) of cilia on the forebrain ChP (G & H) and rhombencephalic ChP (I & J) of katnb1^mh102/+ control (G & I) and katnb1^mh102/mh102 mutant (H & J) brains. Scale bars, 25 μm.