ZFIN Image: Tsakoumis et al., 2022, Fig. 6

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Fig. 6

Fig. 6. Relative gene expression of genes involved in ovulation, steroidogenesis and genes of the leptin system in the ovaries and fully grown (FG) follicles of lepr+/+ and lepr−/− adult female zebrafish. Both for ovaries (n = 6) and for FG follicles (n = 3–6) data are shown as mean ± SE. The expression levels of the target genes were normalized to the expression levels of ef1a. Significant differences between different groups are indicated by *p < 0.05, **p < 0.01, ***p < 0.001. For the ovaries: empty bars indicate lepr+/+ and filled bars indicate lepr−/− ovaries. For the FG follicles: empty bars indicate follicles from lepr+/+ females, filled bars follicles from lepr−/− females which were not laying eggs and striped bars follicles from lepr−/− females which were laying few eggs.

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Acknowledgments

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Reprinted from Molecular and Cellular Endocrinology, 546, Tsakoumis, E., Ahi, E.P., Schmitz, M., Impaired leptin signaling causes subfertility in female zebrafish, 111595, Copyright (2022) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.