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Fig. 9 Distribution of Otpa-positive cells compared to shh-GFP cells in the adult zebrafish brain. Four transverse section levels in DAPI stain (a-d), Otpa immunostain (a1-d1), GFP immunostain (a2-d2) and merged pictures (a3-d3). (a-a3): telencephalon and preoptic region. (b-b3 to d-d3): three levels of posterior tuberculum and hypothalamus. White arrows in (b1-b2) point to the only shh-GFP cells double-labeled with Otpa. See text for more details. Scale bar = 250 µm. Abbreviations: Dm, medial zone of dorsal telencephalon; Hc-a, caudal zone of periventricular hypothalamus in front of posterior recess; Hd/Hv, dorsal/ventral zone of periventricular hypothalamus; IN, intermediate hypothalamic nucleus; LH, lateral hypothalamic nucleus; Nmlf, nucleus of medial longitudinal fascicle; PG, preglomerular complex; PM, magnocellular preoptic nucleus; PTN, posterior tuberal nucleus; PVO, paraventricular organ; TPp, periventricular nucleus of posterior tuberculum; Vi, intermediate nucleus of ventral telencephalon; ZLI, zona limitans intrathalamica