ZFIN Image: Li et al., 2022, Fig. 6

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Figure Caption

Fig. 6

E2 enhances neutrophil apoptosis through suppressing the expression of hif1α and c-myb under physiological conditions.

A E2 decreased neutrophils in AB zebrafish embryos. (t-test, ***p < 0.001, n > 20). B May–Grunwald–Giemsa staining of whole KM blood cells in 6-month-old AB zebrafish after 4 days of E2 treatment (t-test, ***p < 0.001, n = 12). Red arrowheads, blue asterisks, black arrowheads and yellow lightning indicates neutrophils, precursors, lymphocytes and macrophages, respectively. C Staining of hif1α with antibody with or without E2 treatment. D E2 exposure decreased c-myb in the CHT, as determined by WISH. E qPCR quantification of decreased c-myb expression in lyz:Dsred⁺ cells by E2 (t-test, mean ± SEM. ***p < 0.001, **p < 0.01, ^*p < 0.05, n > 20). F Effect of E2 on neutrophil proliferation in AB zebrafish embryos. (one-way ANOVA (LSD). ns, no significance, n > 10). G E2 promotes the apoptosis of myeloid lineage cells in AB zebrafish embryos (one-way ANOVA (LSD) **p < 0.01. n > 10).

Acknowledgments

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