ZFIN Image: Li et al., 2022, Fig. 3

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Figure Caption

Fig. 3

E2 decreases neutrophils in c-myb^hyper transgenic zebrafish embryos through ERs-independent mechanisms.

A–D Knockdown of esr1 (A), esr2a (B), esr2b (C) and gper1 (D) did not alleviate E2-mediated inhibition of c-myb^hyper zebrafish neutrophils. (one-way ANOVA (LSD) ***p < 0.001, **p < 0.01, *p < 0.05. ns, no significance, n > 15). E PPT, DPN and G1 had no effect on c-myb^hyper zebrafish neutrophils. (one-way ANOVA (LSD), ns, no significance, n > 20). F Knockdown of esr1, esr2a and esr2b simultaneously did not alleviate E2-mediated inhibition of c-myb^hyper zebrafish neutrophils. (one-way ANOVA (LSD) ***p < 0.001, **p < 0.01, ns, no significance, n > 15). G ICI 182780 did not alleviate E2-mediated inhibition of c-myb^hyper zebrafish neutrophils. (one-way ANOVA (LSD) ***p < 0.001, ns, no significance, n > 15). H Knockdown of esr1, esr2a esr2b and gper1 simultaneously did not alleviate E2-mediated inhibition of c-myb^hyper zebrafish neutrophils (one-way ANOVA (LSD) ***p < 0.001, **p < 0.01, ns, no significance, n > 15). I The triple esr1; esr2a; esr2b receptor mutant (esr^-/-) was produced through mating to knock out all three classical nuclear receptors. SB staining showed that the E2-induced neutrophil decrease was also not alleviated in the triple mutant. (t-test, mean ± SEM; ***p < 0.001, *p < 0.05. ns, no significance. n > 10).

Acknowledgments

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