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Fig. 3
Mutation characterization in zebrafish- (A) Genotype screening in DNA extracted from zebrafish, Representative electrophoregrams indicating the sequences of WT, heterozygote and homozygote. (B) qPCR analysis quantification of ngly1 mRNA levels. t-test (ngly1 (+/+) n = 3, ngly1 (−/−) n = 3, ***p < 0.001). (C) WB analysis quantification of ngly1 protein levels. t-test (ngly1 (+/+) n = 3, ngly1 (−/−) n = 3, **p < 0.01). (D) WB analysis with an antibody against ZF ngly1; β-actin was used as a loading control.
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