|
Fig. 2
Expression of fmnl2a in zebrafish brain overlaps with astroglia. a Singe cell sequencing tSNE plots for her4.1 and fmnl2a in adult zebrafish telencephalon. b Amyloid toxicity assay and bulk RNA sequencing results for fmnl2a gene expression. c, d Double immunohistochemical staining (dIHCs) for her4.1: GFP (astroglia) and Fmnl2 in control (c) and amyloid-injected (d) brains with DAPI counterstain. c?, d? individual fluorescent channels for Fmnl2. e, f Higher magnification image showing overlapping Fmnl2 and her4.1-driven GFP. g, h Double channel colocalization analyses of e and f. R Pearson correlation coefficient, ? Spearmann?s rank coefficient. Scale bars as indicated