Fig. 9

Figure 9. Staining of mitochondrial autophagosomes by Mtphagy Dye (red) in ZFL cell (A). Nuclei are highlighted with Hoechst 33342 (blue), scale bar = 25 μm (A). The relative gene expression level of PINK1 and Parkin (B). Western blot analysis of PINK1 and P-AMPK in ZFL cell (C). FA group—FA medium (complete LDF medium with 0.25 mM oleic acid and 0.25 mM palmitic acid); FA + HT group—FA medium containing 50 μM HT, FA + HT + Csa group—FA medium containing 50 μM HT with CC pretreatment. All values are exhibited as mean ± SE. The values of bar graphs with different superscripts (a, b) are significantly different at p < 0.05 (Tukey’s test).