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Figure 5—figure supplement 2

Efficient and specific knockdown of zebrafish Strn3 using morpholinos.

(A) Western blotting of 2-dpf head lysates from STD-MO-injected and MO-strn3-injected wild-type embryos. A ~90 kDa band corresponding to zebrafish Strn3 is reduced in the morphants. (B) Quantification of Strn3 protein level relative to loading control protein, β-actin. Mann–Whitney U-test, n = 4. Data are represented as means ± standard deviation (SD). *p < 0.05.

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