ZFIN Image: Nandamuri et al., 2022, Fig 4

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Figure Caption

Fig 4 Cell proliferation in the optic fissure is not altered in the <italic toggle='yes'>dzip1</italic> mutant.

(A-F) Whole mount immunofluorescence in wild type (A-C) and dzip1^ts294e mutants (D-F) for cell death (green; phospho-histone H3) and nuclei (magenta; TO-PRO-3) at 24 hpf (A, D), 36 hpf (B, E), and 48 hpf (C, F). All images are lateral views of 3-dimensional renderings. White dashed lines mark the boundary of the eye; yellow dashed lines mark the lens. (G-I) Quantification of total number of phospho-histone H3-positive cells within the optic fissure in the eye at 24 hpf, 36 hpf, and 48 hpf. n (embryos) for each genotype shown at the base of the graph. P-values were calculated using Welch’s t-test (M-O). Scale bar: 50 μm. D, dorsal; V, ventral; N, nasal; T, temporal.

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Acknowledgments

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