Fig. 5
Fig. 5 Knockdown of competence factors impairs preplacodal specification.
(A–C) Expression of preplacodal markers at 10.5 hpf in (A) foxi1/gata3 double morphants, (B) tfap2a/c double morphants, (C) foxi1/gata3/tfap2a/c quadruple morphants (4-MO). Note the complete loss of preplacodal markers in C. (D) Expression of krox20, p63, P-smad and sizzled during gastrulation in foxi1/gata3/tfap2a/c quadruple morphants. Morphology of a live quadruple morphant at 30 hpf is also shown. (E) Expression of six4.1 and eya1 in p63 morphants alone or in combination with tfap2a/c-MO or foxi1/gata3-MO. All images show lateral views with dorsal to the right and anterior up, except for the live specimen in (D), which shows a lateral view with anterior to the left.