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Fig. 7 Myofibroblasts are mildly activated upon cardiomyocyte chemoptogenetic ablation. A, Activated fibroblasts (?SMA) and MHC (cardiac muscle) immunostaining before and after ventricular amputation at several time points. In uninjured ventricles ?SMA staining is not detectable. After amputation, ?SMA staining is visible inside the injury but by 30 dpa activated fibroblasts were not detected. B, Tg(myl7:fapdl5-cerulean) hearts injected with control MG-ester and treated with near IR-light, stained for ?SMA and MHC. ?SMA was not detected at any time point. C, In chemoptogenetic ablated hearts, myofibroblasts are weakly activated starting at 5 dpi, until 15 dpi. At least n = 3 for each time point. Q-PCR of cytochrome b, (cybb), collagen (col12a1a and col1a1b), fibronectin (fn1b), and periostin a (postna) in amputated and ablated hearts at 7?days and 5?days (D) and at 20?days (E). Scale bar = 100??m