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Fig. 4 The otofB mutation results in reduced Vglut3 in neuromasts. (A–C) Representative confocal images of 72 hpf (A), 96 hpf (B), and 120 hpf (C) WT neuromasts stained for Vglut3. (D–F) Representative confocal images of 72 hpf (D), 96 hpf (E), and 120 hpf (F) mutant neuromasts stained for Vglut3. (G) Quantification of Vglut3 in WT and OtofB neuromasts (t test, p < 0.001). N = 4 larvae, four neuromasts per larvae for both WT and mutant. (H) Quantification of Vglut3 mRNA transcripts in WT and OtofB larvae (t test, p < 0.001). N = 4 for both WT and mutant. Scale bars = 5 µm.
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