Figure 4

Inhibition of Wnt/β-catenin signaling by IWR-1 affects DA neurons in the ventral diencephalon and hypothalamus. (a–l) Expression analysis of the DA neuron marker th by whole mount in situ hybridization in embryos treated with IWR-1 (right) or DMSO (control, left) during time windows indicated on the left side of each row, and subsequently fixed at 80 hpf. The th expressing DC groups 2–6 are indicated in (a) and (b). The arrowhead in (b,d,f,h,j,l) point at decreased th expression in DC5/6 groups. Dorsal views of heads of larvae, images are Z-projections of image stacks. Scale bar in (b) is 100 µm for all images. Numbers N/N in bottom right corner of each image indicate number of representative phenotypes as shown in image versus total embryos analyzed for this condition.