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Fig. 5 A, B. GABAergic neuron defects in wild-type fish treated with ER/Golgi traffic inhibitor BFA (n = 9; ****P < 0.0001; ns, not significant; one-way ANOVA). C, D. Overexpression of paqr3b mRNA improve the number of GABAergic neurons in 3dpf chd7 mutant fish (n = 7 for chd7−/− and n = 11 for chd7−/− + paqr3b mRNA; ****P < 0.0001; **P < 0.05; One-way ANOVA). Of note, the rescue experiment was performed at 3 dpf given the transient nature of mRNA. E. ERK activation by phosphorylation in chd7−/− mutants was restored to normal levels upon overexpression of paqr3b mRNA shown by Western blot quantification (N = 4; **P < 0.05; One-way ANOVA, N is the number of experimental repeats). Data information: Data are presented as mean ± SEM. Scale bar = 50 μm.