Figure 2
Characterization of the hcn2b−/− mutant. (A) Compared morphology between WT and hcn2b−/− zebrafish mutant at 48 h post-fertilization (hpf): there are no differences in length and pigmentation. (B) Homozygous hcn2b mutants showed a higher mortality rate than controls (4–16% vs. 60–80%). (C) No evident defects were detected during early heart development. Both heart morphology and volume were similar in WT and hcn2b−/− (D). Heart rate showed statistically significant differences between WT and hcn2b−/− larvae at 48- and 56-h post-fertilization. Asterisk indicate statistical significance compared to the control, p < 0.05 (*); p < 0.005 (**).