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FIGURE 2
Fibroblast growth factor 21 inhibits liver cell apoptosis after injury. (A) TUNEL assay performed to assess the level of DNA damage in the damaged liver (nuclei, blue; damaged DNA, red; and liver cells, green). (B) RT-qPCR detection of bax mRNA of three groups at 0, 24, and 48 hpa. (C) RT-qPCR detection of bcl2 mRNA of three groups at 0, 24, and 48 hpa. (D) RT-qPCR detection of p53 mRNA of three groups at 0, 24, and 48 hpa. DMSO group: without Mtz treatment, DMSO was applied. Mtz group: after Mtz treatment, grow naturally. Mtz+FGF21 group: after Mtz treatment, treated with FGF21 standard protein. Significance: **p< 0.01, and ***p< 0.001 vs. DMSO group. Data are expressed as mean ± SEM (n = 40 per group, repeat 3 times for each test). Scale bar = 100 μm.