ZFIN Image: Zhang et al., 2021, FIGURE 3

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FIGURE 3

Figure 3. Defects of definitive hematopoiesis in ltv1^Δ14/Δ14 mutant. (A) WISH of HSPC marker c-myb in the thymus, kidney and CHT at 4 dpf. (B) Representative confocal images of ltv1^Δ14/Δ14; runx1:en-gfp mutants and siblings at 4 dpf. (C) Quantification of runx1⁺ cells of ltv1^Δ14/Δ14; runx1:en-gfp mutants (N = 5) and siblings (N = 5) at 4 dpf. Bars represent means with SD. (D) WISH of HSPC marker ikaros in the CHT and thymus at 4 dpf. (E–I) WISH of erythrocyte progenitor marker gata1 (E), erythrocyte marker αe1-globin (F), macrophage markers mfap4 (G) and csf1ra (H), and neutrophil marker lyz (I) in the CHT at 4 dpf. (J) Sudan Black-stained neutrophils in the CHT at 4 dpf. (K) Quantification of αe1-globin, mfap4, csf1ra, lyz, and SB-positive (Sudan Black) cells in panels (F–J), respectively. Numbers of each group are shown in the respective columns. Bars represent means with SD. (L) WISH of lymphocyte marker rag1 at 4 dpf. Yellow arrowhead: thymus; green arrowhead: kidney. Scale bar: 100 μm.

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Acknowledgments

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