ZFIN Image: Wang et al., 2021, Fig. 2

Image

Figure Caption

Fig. 2 The protein structure of 6 mutant alleles in zebrafish generated by zinc finger, TALEN, or CRISPR-Cas9 gene editing.

A Schematic of Puma wild-type and mutant proteins. (BH3, BH3 domain). B Schematic of Noxa wild-type and mutant proteins. C Schematic of p53 wild-type and mutant proteins. TAD transactivation domain, DNA-binding DNA-binding domain, NLS nuclear location signal, OD Oligomerization domain, BR basic region. D Schematic of Mdm2 wild-type and mutant proteins (p53 binding, p53-binding domain; Acidic, acidic domain; ZF, zinc finger domain; RF, ring finger domain). E Schematic of p63 wild-type and mutant proteins (CT, C-terminal region; p63 mutant transcripts undergo nonsense-mediated decay, NMD). F Schematic of p73 wild-type and mutants. Arrow points out the target site of zinc finger, TALEN, or CRISPR-Cas9 gene editing. Orange indicates the key domain in each protein. Light green bar labels full-length wild-type or in-frame truncated mutant protein and the length of amino acid (aa) sequence is indicated. Red tail indicates out-of-frame part of the truncated protein. The figure is created with BioRender.com.

Acknowledgments

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