ZFIN Image: Zhang et al., 2021, Fig. 3

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Figure Caption

Fig. 3 Krt18-positive BECs contribute to physiological hepatocyte homeostasis in a portion of zebrafish adults (A) Experimental scheme illustrating the stage of 4OHT treatment to triple transgenic line Tg(krt18:CreERT²; lfabp:loxP-STOP-loxP-DsRed; lfabp:Dendra2-NTR) from 10 dpf to 11 dpf and analysis at 1 year. (B) Live images showing the expression of Dendra2 and DsRed in the adult livers at 1 year. Co-immunostaining for Dendra2 and DsRed in DMSO- and 4OHT-treated livers. (C) Fluorescence activating cell sorter (FACS) analysis shows the ratio of DsRed⁺ among Dendra⁺ cells in DMSO- and 4OHT-treated livers. Quantification of the percentage of DsRed⁺ among Dendra2⁺ cells in DMSO- (n=5) and 4OHT-treated (no contribution, n=5; low contribution, n=8; high contribution, n=3) livers at 1 year. Numbers indicate the proportion of larvae exhibiting the expression shown. Asterisks indicate statistical significance: ∗∗p<0.01, and p value was calculated by Student t tests. Scale bars: 100 μm. Data are represented as mean ±SEM. See also Figure S2.

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