Fig. S6
Figure S6. Targeted reporter construct knock-in recapitulates endogenous hoxa11b expression patterns, related to Figure 6 (A) Schematic of hoxa11bTg(hsp70:tdTomato) (hoxa11b:tdTom) locus. The transgene is inserted in the reverse orientation relative to the endogenous promoter. (B) Early axial expression in the tailbud, flank, and spinal cord in lateral view. (C) Expression in early fin buds in dorsal view. (D) At 3 dpf, fin fold cells are labeled by both hoxa11bTg(hsp70:tdTomato) and the Hox13 reporter Tg(2PΔepi:eGFP), while only hoxa11bTg(hsp70:tdTomato) marks the endoskeletal disc. Anterior to top in C, distal to top and anterior to left in D; ED, endoskeletal disc; FF, fin fold; pec, pectoral fin; tb, tail bud; scale bars (B) 500 μm, (C) 125 μm, (D) 100 μm (10 μm inset).
Reprinted from Cell, 184(4), Hawkins, M.B., Henke, K., Harris, M.P., Latent developmental potential to form limb-like skeletal structures in zebrafish, 899-911.e13, Copyright (2021) with permission from Elsevier. Full text @ Cell