Fig. 5 Expression of pituitary hormones (fshb, lhb and gh) in the control (amh+/−) and mutant (amh−/−). (A) Real-time qPCR quantification of fshb, lhb and gh expression (n = 6). (B) Detection of fshb and lhb expression in male pituitaries by fluorescent in situ hybridization. The fshb-expressing cells (green) are indicated by arrows. (C) Expression of fshb and lhb in female pituitaries at 3, 6, 8 and 12 mpf (n = 6). (D) Detection of amh expression in the pituitary and gonads (ovary and testis). * P < 0.05; *** P < 0.001. PG, primary growth follicle; NTC, no template control. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Reprinted from Molecular and Cellular Endocrinology, 517, Zhang, Z., Zhu, B., Chen, W., Ge, W., Anti-Müllerian hormone (Amh/amh) plays dual roles in maintaining gonadal homeostasis and gametogenesis in zebrafish, 110963, Copyright (2020) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.