Figure 4

Figure 4

Global depletion of c3a.1 decreases early neutrophil recruitment to a wound. (A) Experimental setup. 3 dpf c3a.1^+/+ or c3a.1^−/− zebrafish larvae were subjected to wounding by tail transection (dashed line), subsequently stained with Sudan Black B, and the tail region (box) was imaged. (B) Representative images and (C) quantification of neutrophil recruitment following tail transection in c3a.1^+/+ (n = 31, 2 hpw; 32, 8 hpw) and c3a.1-/- (n = 41, 2 hpw; 33, 8 hpw) larvae. (D) Quantification of total neutrophil counts in c3a.1^+/+ (n = 21) and c3a.1^−/− (n = 35) larvae. (E) Quantification of caudal fin regenerate length following tail transection, 24 hpw-72 hpw, of c3a.1^+/+ (n = 20, 24 hpw; 20, 48 hpw; 36, 72 hpw) and c3a.1^−/− (n = 39, 24 hpw; 40, 48 hpw; 36, 72 hpw) larvae. (F) Quantification of caudal fin length during larval development, 4dpf-6dpf, of c3a.1^+/+ (n = 40, 4dpf; 40, 5dpf; 19, 6dpf) and c3a.1^−/− (n = 36, 4dpf; 34, 5dpf; 13, 6dpf) larvae. For C–F, all data are expressed as mean + /− SEM; each dot represents one larva; colors represent results of 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.