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Figure 4—figure supplement 1.

ID: ZDB-IMAGE-200822-26
Source: Figures for Yang et al., 2020

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Figure Caption

Figure 4—figure supplement 1. Hepatic response to brain-LPS challenge still evident after establishment of Kupffer cells and adult vasculature/brain structures in juvenile adults.

(a) Live ex vivo imaging of dissected liver from juvenile adult zebrafish after microinjection of either water vehicle or LPS in the brain at 16–18 hpi. Top, arrows point to macrophages in liver using the mpeg1:GFP transgene. Bottom, arrows point to neutrophils in the liver using the lyz:mCherry transgene. (b) Quantification of macrophage density (# mpeg1:GFP+ cell per field of view, FOV). (c) Quantification of neutrophil density (# lyz:mCherry+ cell per FOV). Scatter plots show uninjected controls, or animals microinjected with water vehicle or LPS in the brain. Each field of view (FOV) equals 0.045 squared mm. Statistical significance was determined by a two-tailed Student’s t-test.

Acknowledgments

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