Figure 1-S1

(a) Schematic showing injection of substances (yellow) in the tectum parenchyma (blue) in the four dpf zebrafish larva and subsequent analysis of the periphery that highlights the most prominent change in the liver (red). A, anterior; P, posterior; D, dorsal; V, ventral. (b) In the four dpf zebrafish, macrophages (mfap4+) are normally absent in the liver (dotted region) while sparsely spread throughout the body (arrowheads) as shown in larvae which were uninjected or water vehicle injected in the brain at six hpi. By contrast, after LPS injection in the brain, strong expression of macrophage marker mfap4 is found in the liver (dotted region), while the brain injection site is often also accompanied by a mfap4 expression increase (arrow). (c) Analysis of zebrafish macrophage-specific markers mfap4 and irg1 in the liver (dotted region) after brain injections with water vehicle, E. coli, or LPS. Results indicate that both LPS and E. coli injections induce ectopic localization of macrophages in the liver, which appear to be activated (expressing irg1) at least in a subset, but no macrophages in the liver in control animals.