ZFIN Image: Ibrahim et al., 2020, Fig. 3.

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Figure Caption

Fig. 3.

The efficiency and effects of the CETI-PIC3 systemarepreserved in subsequent generations. (A) Embryos from an F3 generation of the CETI-PIC3 line were crossed to the Tg(ins:cre) line. β cell-specific induction of H2B-GFP was observed and Tnfa staining was enriched within β cells. (B) qRT-PCR analysis demonstrates increase in tnfa transcript levels in this generation of embryos. n=3. *P<0.05 (parametric t-test). (C) The percentage of transgenic embryos from each generation, up to the F3 generation. (D) Quantification of Insulin⁺ H2B-GFP⁺ double-positive cells and Insulin⁺ H2B-GFP⁻ single-positive cells in ins-CETI-PIC3 embryos from F1 and F3 generations to show how responsive CETI-PIC3 embryos are to insulin:cre.

Acknowledgments

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