Fig 1

Fig 1

(A-D) Stereo and confocal microscope images of regenerating fins in wildtype (A,C) and mdka^mi5001 (B,D), immunolabeled with PCNA (magenta). Red lines (A,B) indicate the plane of cross section shown in panels C and D. White lines across the fins show the levels of the amputation plane (C,D). Scale bars equal 400 μm in (A,B) and 50 μm in (C,D). (E-H) Confocal images of the blastemal compartments of fins from wildtype (E,G) and mdka^mi5001 (F,H), immunolabeled with PCNA (E,F) or BrdU (G,H) incorporated between 3–4 dpa (see Materials and Methods). Scale bars equal 50 μm in (E-H). (I) Graph illustrating the average number of BrdU-positive cells within a 400 mm linear distance. wildtype: 186.8 ± 15.1 cells, mdka^mi5001: 142.9 ± 12.3 cells, Student’s t-test, p = 0.0326, n = 5 wildtype and 5 mutants.