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Figure 1 (A, B) ET4;ET20 larvae were treated with neomycin, LY411575 (3dpf-5dpf), or neomycin following LY (LY+neo), and collected at indicated time points post neomycin treatment for sox2 immunostaining. The number of sox2+ progenitors was not affected post neo, while it was significantly decreased in LY and LY+neo-0h. The sox2+ progenitors were regenerated post LY+neo and recovered to normal level at 48 hr post LY+neo. (C, D) The sox2:GFP reporter was treated with LY from 3dpf to 5dpf to exhaust GFP+ progenitors. GFP+ progenitors cannot be regenerated when resting in normal medium for 2 days post LY treatment (LY+rest). In contrast, sox2+ progenitors were quickly recovered to normal level at 2-day post LY+neo. Scale bar equals 10 μm. All groups are compared with 5dpf unless indicated