Figure 5—figure supplement 1.

(A) Top illustration is experimental time line. Bottom graph is qPCR quantification of tgfb1bgene expression. (B) Top illustration is experimental time line. Bottom graph is qPCR analysis of the effect of Tgfb1b expression on tgfb2b and tgfb3 expression. (C) tgfb1b-targeting MO affects splicing of the primary tgfb1b transcript as indicated by the larger PCR fragment identified when embryos were injected with tgfb1b-targeting MO. (D) EdU click-it chemistry was used to identify proliferating MG at four dpi in retinas +/- MO mediated Tgfb1b knockdown. Left panels are representative images and right panel is quantification of data in (D). (E) Quantification of EdU-based lineage tracing of MG-derived progenitors that were labelled with EdU at four dpi and assayed for cell type specific marker at 14 dpi. Zpr1 detects cone photoreceptors; GS detects MG, HuC/D detects amacrine cells in INL and ganglion cells in GCL.