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Fig. 8 Disrupted smooth muscle development contributes to intestinal dysgenesis in uhrf1 mutants. Confocal images of 5 dpf intestines labeled with smooth muscle myosin (SMM) and desmin (magenta) antibodies. Dissected intestines (A–D) of wildtype (A,C) and uhrf1 mutants (B,D). In both proximal (B) and distal (D) intestine, uhrf1 mutants essentially lack smooth muscle cells, as revealed by whole-mount antibody staining. Transverse sections of wildtype (E,G) and uhrf1 mutants (F,H) showing loss of intestinal smooth muscle in mutants. White arrows point to phox2b:EGFP (green) positive ENS neurons that are further removed from the intestinal epithelium in mutants (F,H) than in wildtypes (G). Brightfield images of transverse sections of 5 dpf wildtypes (I,K) and uhrf1 mutants (J,L) stained with hematoxylin and eosin show disrupted proximal and distal intestinal development in mutants compared to wildtypes. Increased shedding of cells in uhrf1 mutants is indicated by a yellow arrow and disrupted intestinal epithelium with a blue arrow. Higher magnification insets of boxed areas indicate disrupted intestinal epithelium morphology in uhrf1 mutants. ie = intestinal epithelium. Scale bar = 50 μm in A-D, and 25 μm in E-L.
Reprinted from Developmental Biology, 455, Ganz, J., Melancon, E., Wilson, C., Amores, A., Batzel, P., Strader, M., Braasch, I., Diba, P., Kuhlman, J.A., Postlethwait, J.H., Eisen, J.S., Epigenetic factors Dnmt1 and Uhrf1 coordinate intestinal development, 473-484, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.