Fig. s7

Fig. s7 Cell proliferation and cell death are not affected in the pharyngeal arches with loss of prdm16. (A-D) Wildtype or prdm16−/− zebrafish embryos crossed into the Tg(sox10:EGFP) transgenic line were collected at 24 hpf and whole mount immunofluorescence was performed for phosphorylated histone H3 (A) or cleaved caspase 3 (B) to assess cell proliferation or apoptosis in the pharyngeal arches, respectively. Shown are lateral max projection images of the pharyngeal arches 1–7. (C-D) Quantification of the number of phosphorylated histone H3 positive cells (yellow) (C) or cleaved caspase 3 positive cells (yellow) (D) within the pharyngeal arches (n ​= ​6 embryos for each group). Abbreviations: eye (e), pharyngeal arches (1–7). Scale bar 100 ​μm ​ns, not significant, Student’s t-test.